Crystallization (Comment) | Organism |
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vapor diffusion method is used to obtain crystals with a rod-like morphology of a maximum size of about 0.4 x 0.2 x 0.2 mm3. Crystal structure of the heterodimeric bienzyme ImGP synthase comprising the glutaminase subunit HisH and the cyclase subunit HisF, at 2.4 A resolution. | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X0C8 and Q9X0C6 | Q9X0C8: subunit HisH, Q9X0C6: subunit HisF | - |
Thermotoga maritima DSM 3109 | Q9X0C8 and Q9X0C6 | Q9X0C8: subunit HisH, Q9X0C6: subunit HisF | - |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate | the enzyme catalyzes two different reactions at two separate active sites. At the glutaminase active site, glutamine is hydrolyzed to glutamate and ammonia, which, at the synthase active site, reacts with the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate. Ammonia is channeled between glutaminase and synthase active sites | Thermotoga maritima | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate | the enzyme catalyzes two different reactions at two separate active sites. At the glutaminase active site, glutamine is hydrolyzed to glutamate and ammonia, which, at the synthase active site, reacts with the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate. Ammonia is channeled between glutaminase and synthase active sites | Thermotoga maritima DSM 3109 | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ImGP synthase | - |
Thermotoga maritima |
General Information | Comment | Organism |
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metabolism | key metabolic enzyme,which links amino acid and nucleottide biosynthesis. It uses the substrates glutamine and N'-[(5-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide to catalyze the closure of the imidazole ring within histidine biosynthesis and additionally provides 5-aminoimidazole-4-carboxamide ribotide for use in the de novo synthesis of purines | Thermotoga maritima |